The primary and foremost modes of transmission of bacterium are our hands. Hand hygiene is the single most important, simplest, and least expensive means of preventing nosocomial infections Proper Hand Washing is the most important means of preventing the spread of infection. Hence it brings us to the use of antiseptic for hand washing purposes. The present research was aimed to evaluate the anti- microbial efficacy of herb and plant material such as Mint (Mentha sp.)  and Azadirachta indica by agar-plate diffusion method of these plant extracts that were obtained by soxhlet extraction. Also the research was carried out to formulate and evaluate the herbal hand wash liquid containing the above extracts. The anti-microbial activity of the formulated hand wash gel was tested against E. coli and S. aureus by Agar plate diffusion method. Thus this work suggests and supports the incorporation and utilization of herbs and traditional plant materials in the formulation to give better anti-microbial effect. Results revealed that Mint containing soap formulation was more efficient in reducing the number of organisms from hands thus it can be used as an antiseptic soap with less or no side effects.