In this article potential antimicrobial and antioxidant activities of the selected herbal extracts obtained from twelve plants from their different parts (leaf, stem, bark, seed and fruit) that have been traditionally used as general health supplements in Myanmar. The aim of this current research was to evaluatate the antimicrobial and antioxidant potential of ethnolic crude extracts of all these plants, as well as their total phenol contents. In vitro antimicrobial activity was evaluated against six strains; Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Enterococcus faecalis. by agar well diffusion method. Among all of these plant extracts, the extract of Cassia fistula was strongly inhibited against Enterococcus faecalis. The most antioxidant activities against DPPH were displayed by the extract of Celastras paniculata Wild. and Piper nigrum exhibiting 88.38% and 85.43% inhibition respectively. And the highest value of reducing power was observed by the extract of Celastras paniculata Wild.. and followed by Cassia fistula. Similarity, the highest amount of total phenolic contents was found in Cassia fistula. The brine shrimp lethality assay was also obsoleted to examine the toxic potential of plant extracts. Based on the above results, it can be concluded that the plant extract of Cassia fistula have greatest potential as a source of natural antioxidant and antimicrobial agents than other plant extracts. In the future, plant-derived bioactive compounds will be an essential aspect of the therapeutic agents to accelerate their future discovery in biomedical and natural product research.