Keratinophilic fungi have been reported as one of the principal causes of cutaneous and sub cutaneous mycoses among individuals living in developing countries. This study was undertaken to determine the diversity, and molecularly characterized keratinophilic fungi from different soil samples in Uli community, Ihiala L.G.A, Anambra State. A total of 60 soil samples were collected randomly from different sites; poultry farm, goat farm and garden, and screened for the presence of keratinophilic fungi using hair baiting technique. The isolates obtained were characterized based on their macroscopic, microscopic and molecular characteristics. Microsporum audouinii strain INI48063 (MAINI), Microsporium gypsuem strain ATCC58595 (MGATCC), Microsporum nanum 5.8S rRNA gene (MNRNA), Trichophyton mentagrophytes strain DSM 108623 (TMDSM), Trichophyton rubrum strain AVMF11 (TRAVMF), Trichophyton megninii strain UAMH8542 (TMUAMH) were isolated from the soil samples, and the organisms were detected most in goat farms. TMDSM recorded significantly (p<0.05) the highest occurrences among the soil samples collected from poultry (9.26%) and goat (20.37%) farms, and TRAVMF recorded was significantly (p<0.05) seen most in the soil samples collected from garden (9.26%). TMUAMH and MNRNA were not detected in soil samples collected poultry farm. This study has shown the occurrences of keratinophilic fungi in the studied soil samples, and TMDSM was mostly seen in the samples.