Biology and Life Sciences
Received: 23 Oct 2018 , Published: 08 November 2018
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|1||Ramesh Chandra Agrawal|
Gymnema is popularly known as gurmar or Madhunashini (destroyer of sugar), as chewing the leaves causes a loss of sweet taste.
Objectives: The aim of this study was to assess the Antiproliferative effects of Gymnema sylvestre leaves extract in In Vitro test systems such as dimethylthiazol diphenyltetrazolium bromide (MTT) Assay, neutral red uptake ( NRU) Assay, DNA fragmentation Assay . Materials and Methods: HEPG2 cells were exposed to 0.1, 1, 10, 100, 1000 µg/ml concentration of Gymnema extract in a 96-cluster-well-culture plate for 24 h. The cell viability after exposure to Gymnema sylvestre was determined by MTT and NRU assays in separate tissue culture plates.
Results: Gymnema sylvestre at concentrations of 0.1, 1.0,10,1000 and 1000 ug/ml showed dose dependent inhibition of HEPG2 cells lines and caused cytotoxicity .
Conclusion:In general, the MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of Gymnema sylvestre
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