Biology and Life Sciences

Biology and Life Sciences

Antiproliferative effect of Gymnema sylvestre in In Vitro assays

Pages: 13  ,  Volume: 15  ,  Issue: 1 , October   2018
Received: 23 Oct 2018  ,  Published: 08 November 2018
Views: 8  ,  Download: 0

Authors

# Author Name
1 Ramesh Chandra Agrawal
2 Sonam Soni

Abstract

Gymnema is popularly known as gurmar or Madhunashini (destroyer of sugar), as chewing the leaves causes a loss of sweet taste.  

Objectives: The aim of this study was to assess the Antiproliferative effects of Gymnema sylvestre leaves extract in In Vitro test systems such as dimethylthiazol diphenyltetrazolium bromide (MTT) Assay, neutral red uptake ( NRU)  Assay, DNA fragmentation Assay . Materials and Methods: HEPG2 cells were exposed to 0.1, 1, 10, 100, 1000 µg/ml concentration of Gymnema extract  in a 96-cluster-well-culture plate for 24 h. The cell viability after exposure to Gymnema  sylvestre was determined by MTT and NRU assays in separate tissue culture plates.

Results: Gymnema  sylvestre at concentrations of  0.1, 1.0,10,1000 and 1000 ug/ml showed dose dependent inhibition of HEPG2 cells lines  and caused cytotoxicity .

Conclusion:In general, the MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of Gymnema  sylvestre 

Keywords

  • MTT assay
  • Gymnema sylvestre
  • References

     

    1. Agrawal, R.C., Jain, R., Wasim Raja, Ovais, M. (2009): Anticarcinogenic Effects of Solanum lycopersicum Fruit Extract on Swiss Albino and C57 BL Mice. Asian Pacific Journal of Cancer Prevention.10:379-381.
    2. Berenblum, J., Shubik, P. (1947): A new quantitative approach to the study of stages of chemical carcinogenesis in the mouse skin. Br. J. Cancer. 1:373-391.
    3. Bhatia AL, Raka K, Gulshan V, Sharma KV, Sharad V, Megha J (2008). Radio protective role of Gymnemic acid on mice: Study on Hepatic Biochemical Alterations. Asian J. Exp.Sci; 22(3):427-32.
    4. Gloria Y Yeh, David M. Eisenberg, Ted J. Kaptchuk, Russell S. Phillips (2003). Systematic Review of Herbs and Dietary Supplements for Glycemic Control in Diabetes. Diabetes Care; 26 (4): 1277-1294.
    5. Jitender KM, Manvi FV, Alagawadi KR and Noolvi M (2008): Evaluation of anti-inflammatory activity of Gymnema sylvestre leaves extract in rats. Int. J. Green Pharmacy: 114-115.
    6. Jitender KM, Manvi FV, Nanjwade BK, Alagawadi KR and Sanjiv S (2009): Immuno - modulatory activity of Gymnema sylvestre leaves extract on in vitro human neutrophils. J. Pharmacy Res; 2(8):1284-6.
    7. Kanetkar, P.V. Laddha, K.S. Kamat. M.Y (2004).  Gymnemic acids: A molecular perspective of its action on carbohydrate metabolism. Poster presented at the 16th ICFOST meet organized by CFTRI and DFRL, Mysore, India.
    8. Kiranmai M, Kazim SM and Ibrahim M (2011). Combined wound healing activity of Gymnema sylvestre and Tagetes erecta Linn. Int. J. Pharmaceu. Appl,; 2(2):135-140.
    9.  
    10. Luo H (1999). Possible participation of NO and EDHF in the relaxation of rat intestinal circular muscle induced by Gymnema water extracts containing gymnemic acids. Journal of the Yonago Medical Association; 50(1): 22-31.
    11. Morse MA, Stoner GD. Cancer chemoprevention: principles and prospects, Carcinogenesis. 1993; 14(9) :1737-1746
    12. Murakami A, Ohigashi H, Koshimizu K. Anti-tumor promotion with food phytochemicals: a strategy for cancer chemoprevention, Bioscience, Biotechonolgy,and Biochemistry.1996; 60(1) :1-8,
    13. Ohmori, R Iwamoto, T Tago, M Takeo, T (2005). Unno, T Itakura H.  Lipids, 40 (8), 849-53.
    14. Pitot HC, Dragan YP, Facts and theories concerning the mechanisms of carcinogenesis, The FASEB Journal.1991; 5(9) :2280-2286
    15. Preuss, H.G. Bagchi, (2004).  D Bagchi, M.. Rao, C.V. Dey, D.K Satyanarayana. S. Diabetes Obes Metab, , 6(3), 171-80.
    16. Rachh PR, Patel SR, Hirpara HV, Rupareliya MT, Rachh MR, Bhargava AS, Patel NM and Modi DC (2009): In-vitro evaluation of antioxidant activity of Gymnema sylvestre R.Br.leaf extract. Rom. J.Biol.Plant Biol,; 54(2):141-8.
    17. Sawabe Y, Nakagomi K, Iwagami S, Suzuki S, Nakazawa H (2009). Inhibitory effects of pectic substances on activated hyaluronidase and histamine release from mast cells. Biochim Biophys Acta 1992; 1137(3): 274-278.
       
    1. Sporn MB, Approaches to prevention of epithelial cancer during the preneoplastic period, Cancer Research.1976;36(7): 2699-2702,
    2. Srikanth AV, Sayeeda M, Lakshmi NM, Ravikumar P and Madhava RB (2010): Anti cancer activity of Gymnema sylvestre R.Br. Int. J. Pharmaceu. Sci. Nanotech; 3(1):897-899.
    3. Tandon Pankaj, Sirohi Anita (2010). Assessment of larvicidal properties of aqueous extracts of four plants against Culex quinquefasciatus larvae. JJBS; 3(1):1-6.
    4. Walter BM, Maria CDN, Bettina MRP and Nuno AP (2000). Plant natural products active against snake-bite-the molecular approach. Phytochemistry; 55:627- 642.