Groundwater remains a major natural water resource tapped for drinking and other economic activities across the globe. This resource, however, cannot be optimally used and sustained unless there is constant and frequent assessment to guarantee its quality for consumption. In this study, groundwater quality and status in seven locations in Owerri was assessed. It was aimed at the assessment of groundwater quality from domestic boreholes in parts of Owerri, Imo State. Fourteen (14) water samples, two from each location, were collected from boreholes in Eziobodo (B1), Obinze (B2), Nekede (B3), World Bank (B4), Egbu (B5), Orji (B6) and Amajeke (B7). The physicochemical and biological parameters of these samples were investigated using standard laboratory techniques. Spatial distribution maps of SO42-, total coliform count, turbidity, TSS, NH3, and pH were generated using Geographical Information System (GIS). The values of the physicochemical parameters were juxtaposed with the World Health Organization (WHO) values. The results, relative to recommended standards by WHO, revealed that most of the groundwater samples were contaminated. This paper presents a pivotal contribution for understanding the relationship between land use and groundwater quality in some parts of Owerri.