Introduction: Burn injuries represent a significant global health concern, with high prevalence and mortality rates worldwide, including in Indonesia. Current treatment methods for burns face challenges such as antibiotic resistance and limited regenerative techniques, necessitating innovative solutions. Physeter Macrocephalus ambergris has emerged as a promising candidate due to its anti-inflammatory and antibacterial properties, potentially aiding in wound healing. Methods: A true experimental design was employed to investigate the effect of topical application of Physeter Macrocephalus ambergris on second-degree burn wounds in a rat model. Four groups were studied over 21 days, with histological evaluations performed. Statistical analysis was conducted using univariate analysis and ANOVA/Kruskal-Wallis tests. Results: Physeter Macrocephalus ambergris oil significantly promoted epithelialization compared to controls on both the 14th and 21st days. Neutrophil levels were significantly reduced in the ambergris-treated group compared to the negative control, indicating improved wound healing. However, no significant differences were observed between ambergris-treated groups and those treated with NaCl or vaseline. Discussion: Physeter Macrocephalus ambergris oil demonstrated a notable effect on epithelialization and wound closure, potentially attributed to its bioactive compounds. Faster re-epithelialization was observed, suggesting accelerated wound healing. The study underscores the potential of ambergris oil as a novel modality for burn treatment.

burns

physeter macrocephalus ambergris

wound healing

epithelialization

animal model