Background : The harmful content contained in cigarettes smoked will cause damage to various organs and cells , especially the leydig cells as the main producer of the hormones testosterone . Vitamin D in the human body has a pleiotropic effect and functions in cell differentiation and proliferation . this study aims to prove that the number of leydig cells and testosterone levels in the group given orally vitamin D were higher than the control group in male wistar rats ( Rattus Novergicus ) exposed to cigarette smoke. Methods : This study used a randomized posttest only control group design . The subjects were 36 white rats , Wistar strain , male, healthy , aged 3-4 months , weight 180-200 grams which were divided into two groups (18 rats / group ). The controls group was given a placebo (1 ml of distilled water ) and exposed to cigarettes smoke , the treatments group was given oral vitamin D 0.5 mg / kg / day in 1 ml distilled water ( aquadest ) and exposed to cigarettes smoked for 30 minutes every day before treatments for 21 days . After treatment , the number of Leydig cells was examined histopathologically by HE staining and the testosterone level was examined by the ELISA method . Results : The results showed that the mean number of Leydig cells in the treatments group was higher , 12.1 ± 2.88 cells / field of view , whereas in the control group it was lower , 5.05 ± 2.19 cells / field of views (p = 0.001). Testosterone levels in the treatments group were also higher , 21.2 ± 4.0 nmol /L, while those in the control group were 8.9 ± 1.1 nmol /L (p = 0.001). Conclusion : It can be concluded that oral vitamin D can prevented aging by increasing the number of Leydig cells and testosterone levels in male Wistar rats ( Rattus novergicus ) exposed to cigarettes smoked .  

testosterone

VITAMIN D