Background: Pseudomonas aeruginosa have a significant proportion of the causes of bacterial keratitis. Corneal ulcers infected with P. aeruginosa are more severe than other bacterial corneal ulcers. P. aeruginosa is often associated with keratitis that results from wearing contact lenses, which eventually leads to corneal ulcers. This study aims to determine the differences in the results of the antibiotic sensitivity test of P. aeruginosa from corneal scraping specimens of keratitis patients using contact lenses and non-contact lens users at RSUD dr. Soetomo. Methods: This study is an observational analytic study of secondary data from corneal scraping cultures of patients with keratitis infected with P. aeruginosa at Dr. Soetomo Hospital period January 2017-December 2020. Results: The total number of samples included in the inclusion criteria was 46 samples. Of the total sample, 8 (17.40%) were contact lens users and 38 (82.60%) were non-contact lenses. The male sex and female sex each as much as 50%. The distribution of sample age categories for 0-20 years was 21.74%, 21-40 years was 13.04%, 41-60 years was 41.30%, and >60 years was 23.91%. Based on samples from 8 contact lens patients, antibiotics were found to be 100% sensitive, namely Amikacin, Cefepime, Ciprofloxacin, Imipenem, Levofloxacin, Meropenem, and Piperacillin tazobactam, Aztreonam and Gentamicin 87.5%, Ceftazidime 75%, Tobramycin 37.5% and Piperacillin 25%. Meanwhile, from a sample of 38 patients who did not wear contact lenses, the sensitive antibiotics were Cefepime 100%, Meropenem 94.8%, Amikacin 92.1% Imipenem 86.9%, Piperacillin-tazobactam 76.3%, Ciprofloxacin 73.8%, Gentamicin 73.8%, Aztreonam 68.4%, Levofloxacin 68.4%, Piperacillin 50%, Ceftazidime 44.7%, and Tobramycin 28.9%. Conclusion: The results of the antibiotic sensitivity test against P. aeruginosa from keratitis patients with a history of contact lens use showed that 8/8 (100%) of the active antibiotics were Amikacin, Cefepime, Ciprofloxacin, Imipenem, Levofloxacin, Meropenem, and Piperacillin tazobactam. Then followed by Aztreonam and Gentamicin (7/8). The results of the antibiotic sensitivity test against P. aeruginosa from keratitis patients who had no history of using contact lenses showed that the active antibiotics were above 50%, namely Cefepime 38/38 (100%), Meropenem 36/38 (94.8%), Amikacin 35 /38 (92.1%), Imipenem 33/38 (86.9%), Piperacillin-tazobactam 29/38 (76.3%), Ciprofloxacin 28/38 (73.8%), Gentamicin 28/38 (73 .8%), Aztreonam 26/38 (68.4%), and Levofloxacin 26/38 (68.4%). Keywords: Antibiotic sensitivity, Pseudomonas aeruginosa, keratitis, contact lenses

pseudomonas aeruginosa

keratitis

contact lenses

antibiotic sensitivity