Cosmetic products based on traditional Burmese beauty known as ?Myanmar Thanakha? are recently very popular among Asian-wide cosmetic market. In this study, nanoemulsion was formulated from Thanakha (Hesperethusacrenulata) extract, olive oil and nonionic surfactant Tween 80 by low-pressure homogenizer and ultrasonicator techniques. The resulting nanoemulsion was creamy colour in appearance and its morphology was identified by transmission electron microscopy (TEM). Transparent nanoemulsion with mean droplet diameter of 58.23 nm was identified by dynamic light scattering (DLS) method. The resulted pH value of Thanakha nanoemulsion was 5.3 and it was compatible to skin pH (4.5?6.0); a poly dispersion index (PDI) was 0.28 and zeta potential value was ?28.9. Antibacterial activity of formulated nanoemulsion was tested by agar-well diffusion test against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and Escherichia.coli bacteria and the inhibition zone area against skin infective Staphylococcus aureus bacteria was the widest among other. The sun protection factor (SPF) of formulated nanoemulsion was also tested by UV spectrophotometer and the result showed that it has low UV protective activity.