Introduction:There are no standard criteria defining how prominent or how frequent the nucleoli must be before they are sufficient to warrant a diagnosis of high-grade PIN. Histopathology remains as the gold standard for diagnosis of these putative precursor lesions of prostatic carcinoma. Identification of these lesions of prostate helps in early detection of carcinoma and guides the urologist to give an appropriate management to the patient. Methods: This is diagnostic test study with cross sectional design. It was conducted at Anatomical Pathology Department in Universitas Sumatera Utara, Medan, Indonesia. There were total 55 cases taken for this study. AgNORs staining was evaluated in all cases. Results: There is a significance difference between premalignant and malignant lesions that were stained by AgNORs (p value <0,001). The AgNORs value of malignant lesions is higher than premalignant lesions. Conclusion: Histochemical AgNORs staining can help in confound and hesitate cases to distinguish premalignant and malignant of the prostate lesions.