This study assessed ameliorative effect of Virgin Coconut Oil (VCO) following atrazine-induced metabolic derangement in rats. Adult male albino Wistar rats weighing 180-200g body weight were used for the study. They were randomly separated into two major experimental groups (The test and recovery groups). Thirty-five (35) rats in the test group were randomly divided into five sub-groups of 7 rats per sub-group (n=7) and were treated thus: Subgroup (SG) 1 served as normal control and received 10ml/kg body weight of distilled water, SG 2 received 10ml/kg of VCO, SG 3 received 123mg/kg of Atrazine (ATZ), SG 4 was the diabetic control that were left untreated and SG 5 was the diabetic group that were treated with 10ml/kg of VCO. Treatment in the test group lasted for 2 weeks, after which the animals were sacrificed and blood collected for analysis. During these 2 weeks? period, thirty-five rats for the recovery group were also divided into 5 subgroups of 7 rats per sub-group (n=7) and were treated as follows: SG 1 served as normal control and received 10ml/kg body weight of distilled water, SG 2 received 10ml/kg of VCO, SG 3, 4 and 5 received 123mg/kg of ATZ. After 2 weeks, the animals were re-treated for recovery and were treated thus: SG 1 received 10ml/kg body weight of distilled water, SG 2 received 10ml/kg of VCO, SG 3 received 123mg/kg of ATZ, SG 4 was treated with 10ml/kg of VCO and SG 5 was given 10ml/kg of distilled water. After 2 weeks, the animals were also sacrificed and blood collected for analysis. In the test groups, on oxidative enzymes; Glutathione (GSH), Superoxide Dismutase (SOD) and Catalase (CAT) levels were significantly reduced (p<0.05) in the atrazine and diabetic groups when compared to normal control. Following recovery, GSH was significantly increased (p<0.05) in the VCO recovery group when compared to ATZ group. In conclusion, ATZ toxicity caused oxidative stress but its withdrawal significantly reduced the stress; with more pronounced effect following VCO administration.