Biology and Life Sciences

Biology and Life Sciences


Pages: 14  ,  Volume: 40  ,  Issue: 1 , November   2019
Received: 07 Nov 2019  ,  Published: 21 November 2019
Views: 44  ,  Download: 35


# Author Name
1 Zakaria Ahmed
2 Taslima Rahman
3 Sanchita Sarkar


The present study was conducted for antimicrobial activity assessment of five selected fresh samples from different conditions. The antimicrobial effects of Tripfala, Java plum, Cinnamon, Henna and Cumin against both gram-positive and gram-negative bacteria were investigated using the agar well and disc diffusion method.  Aqueous extracts of selected samples extracts showed larger zone of inhibition or antimicrobial activity than ethanolic extracts. Among the five selected microbes Klebsiella sp. showed lowest sensitivities against Tripfala, Java plum whereas these aquous and ethanolic extracts showed great antimicrobial activities against four other selected microbes. All of the natural products showed the MIC values ranged from 12.5-25.0 ug/ml while the MBC values ranged from 25- 100.0 ug/ml. No change of antimicrobial activity was found during four months preservation of prepared discs of these natural plant products. This study is an indication that the Tripfala and Java Plum has the potentiality to use as a source for new broad spectrum oral antibiotics.



Ayurvedic Formulary of India, Part I, 2nd English ed. New Delhi: Controller of Publications; 2003.

Beuchat LR and Golden DA. 1989. Antimicrobials Occurring Naturally in Foods. Food Technology, 43, 134-42.

Brkovi? LD, Ljiljana ?, Slavica SS. 2006. Antibacterial activity of some plants from family Apliaceae in relation to selected phytopathogenic bacteria. Kragujevac J. Sci. 28, 65-72.

Cowan MM. 1999. Plant products as antimicrobial agents. Clin Microbiol Rev. 12(4): 564-82.

Fariba B, Hassan R and Homeyra E. 2010. In vitro study of the effects of henna extracts (Lawsonia inermis) on Malassezia species. Jundishapur J Microbiol.  2010(3): 125–8.

Fabricant DS and  Farnsworth NR.  2001. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 109 (Suppl 1): 69–75.

Ghani A. 1998. Medicinal Plants of Bangladesh- Chemical Constituents and Uses. Asiatic Society of Bangladesh, Dhaka. 215- 216

Graul T, Cain AM and Karpa KD. 2009. Lactobacillus and bifidobacteria combinations: a strategy to reduce hospital-acquired Clostridium difficile diarrhea incidence and mortality. Med Hypotheses. 73(2): 194-8.

Geissman TA. 1963. Flavonoid, Compound, Tannis, Lignins, and compounds. M. Florkin, E.H. Stotz (Eds.), Pyrrole Pigments, Isoprenoid Compounds and Phenolic Plants Constitutes, 9, Elsevier, New York.

Höfling, JF, Anibal, PC, Obando-Pereda GA, Peixoto IAT, Furletti VF, Foglio MA and Gonçalves RB. 2010. Antimicrobial potential of some plant extracts against Candida species. Braz. J. Biol., 70(4), 1065-68.

Hawkey PM. The origins and molecular basis of antibiotic resistance. 1998. BMJ, 317(7159):657-60.

Ibukun A, Tayo A, Toyin A, Tolu O, and Tolu O. 2007. Evaluation of the Antimicrobial Properties of Different Parts of Citrus Aurantifolia (Lime Fruit) as Used Locally. Afr J Tradit Complement Altern Med. 4(2): 185–90.

Kapadia SG and Yagnik BN. 2013. Current Trend and Potential for Microbial Biosurfactants. Asian J Experimental Biol Sci, 4, 1-8.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. 1999. Eds. Manual  of  clinical microbiology.  7th ed.    Washington, DC: ASM.

Nayak BS, Isitor G, Davis EM and Pillai GK. 2007. The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res. 21(9): 827–31.

Pan SY, Gao SH, Zhou SF, Tang MK, Yu ZL and Ko KM. 2012. New perspectives on complementary and alternative medicine: an overview and alternative therapy. Alter Ther Health Med. 18: 20–36.

Pradhan SJ, Nesler KR, Rosen SF, Kato Y, Nakamura A, Ramaswami M and Barbee SA. 2012. The conserved P body component HPat/Pat1 negatively regulates synaptic terminal growth at the larval Drosophila neuromuscular junction.  J. Cell Sci. 125(24): 6105--6116.

Sukanya SL, Sudisha J, Hariprasad P, Niranjana SR, Prakash HS and Fathima SK. 2009. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. African Journal of Biotechnology, 8 (23), 6677-82.

Srikumar R.  N. Jeya Parthasarathy E. M. Shankar S. Manikandan R. Vijayakumar R. Thangaraj K. Vijayananth R. Sheeladevi Usha Anand Rao. 2007. Evaluation of the growth inhibitory activities of triphala against common bacterial isolates from HIV infected patients. Phytotherapy Research. 21(5),  476-80.

Tahsina SH, Mohammad AH, Mohammad GM, David JB, Masayuki F, and Lam-Son P. 2016. Tran. Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance. Front Plant Sci.; 7: 1341.

Wanner J, Schmidt E, Bail S, Jirovetz L, Buchbauer G, Gochev V, Girova T, Atanasova T and Stoyanova A. 2010. Chemical composition, olfactory evaluation and antimicrobial activity of selected essential oils and absolutes from Morocco. Nat Prod Commun. 5(9):1349-54.

Winslow LC and Kroll DJ. 1998. Herbs as medicines. Arch Intern Med. 158(20): 2192-9.