Medicine, Health & Food
Received: 03 Mar 2019 , Published: 18 March 2019
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Single cell protein (SCP) are microbial cells or a mixed culture grown in mass culture and harvested for use as protein source for food and animal feed using agricultural wastes as substrates. The production of single cell protein (SCP) using pineapple and banana peel wastes as substrates in the presence of different carbon and nitrogen sources by a local isolate of Trichoderma viride was investigated. The methods employed were proximate analysis, growth determination using mycelia dry weight, absorbance (sporulation) and pH as parameters whereas protein yield determination was done using proximate protein analysis. The result revealed that the nutrient found in pineapple and banana peel extracts were 7.43 % and 9.04 % of crude protein, 6.72 % and 7.68 % of fibre, 5.04 % and 6.61% of fat, 40.12 % and 48.16 % of carbohydrate, 5.84 % and 6.15 % of ash. The highest and lowest values for pineapple and banana media supplemented with carbon sources using sodium nitrate as nitrogen sources were cellulose (3.28 g) – Glucose (0.45 g) and cellulose (3.05 g) – fructose (0.44 g) mycelial dry weight; maltose (3.620) – mannose (0.275) and saccharose (7.590) – maltose (0.918) for absorbance; cellulose (7.08) – glucose (6.18) and cellulose (7.24) – maltose (6.20) for pH and fructose(18.35 %) – glucose (9.57 %) and saccharose (30.96 %) – sucrose (13.20 %) for protein yields. Also, the media supplemented with nitrogen sources using sucrose as carbon source revealed: potassium nitrate (1.13 g) – ammonium oxalate (0.62 g) and sodium nitrite (1.04 g) – potassium nitrate (0.71 g) for mycelial dry weight; ammonium oxalate (1.288) – sodium nitrite (0.200) and ammonium oxalate (2.643) - ammonium sulphate (0.155) for absorbance; ammonium oxalate (6.61) – ammonium nitrate (5.99) and ammonium oxalate (6.57) – potassium nitrate (6.25) for pH and potassium nitrate (20.31 %) – ammonium nitrate (9.20 %) and sodium nitrate (27.72 %) – ammonium nitrate (18.04 %) for protein yield on pineapple and banana extracts, respectively after 5 – 7 days incubation period. Banana extract protein yield increased significantly at p (< 0.05) than pineapple extract. There was no significant differences detected in the biomass content of both banana and pineapple extracts. The present findings reveal that both banana and pineapple wastes could be used as effective alternative carbon and energy source for SCP production but banana offers a better option. Thus, the potential of Trichoderma viride to consume the substrates could also be exploited for effective waste management.
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